Inhibitory effect of carbamates in two types of cholinesterases

Abstract

Inhibitory effect of substituted 3-(diethylaminophenyl) phenyl carbamates in two types of cholineserases (acetylcholinesterase and butyrylcholinesterase) was determined. Inhibiting effectiveness was described by the value of IC50 (50 % inhibitory concentration). Modified spectrophotometric Ellman's method was used. Two types of choline esters were used as substrates for cholinesterases. It was proved that the carbamates chosen are quite effective inhibitors of cholinesterases. The most effective inhibitor of acetylcholinesterase is 3-(diethylaminophenyl) phenyl carbamate with the value of IC50 = 1.52 μM and the most effective inhibitor of butyrylcholinesterase is 3-(diethylaminophenyl) (3- nitrophenyl) carbamate with the value of IC50 = 1.70 μM.