Principles of membrane permeabilization of cells and organelles

Abstract

This thesis deals with the cellular and organellar membrane permeabilization and its occurence in physiological cell death and some pathophysiological processes with closer attention to cancer. Laboratory induced membrane permeabilization has found its importance within the development of treatment and better understanding of mechanisms staying behind certain diseases. Methods and their principles used to effectively disrupt the phospholipid bilayers in case of laboratory analysis has been described. Based on the increasing knowledge in the field of molecular biology, better treatment and early diagnostics of diseases can be achieved. This thesis helps to understand principles staying behind the cellular and organellar membrane permeabilization and analytical approaches used within the framework of evolving molecular analysis and cellular treatment.