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Publikace:
Voltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particles

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dc.contributor.authorČadková, Michaela
dc.contributor.authorKorecká, Lucie
dc.contributor.authorŠálek, Petr
dc.contributor.authorMetelka, Radovan
dc.contributor.authorBílková, Zuzana
dc.date.accessioned2012-03-07T07:44:12Z
dc.date.available2012-03-07T07:44:12Z
dc.date.issued2011
dc.description.abstractPossibility of determination of ovalbumin as a model protein based on ELISA principle is reported in this contribution. Model system consisted of anti-ovalbumin IgG (primary antibody), ovalbumin and anti-ovalbumin-HRP (secondary antibody). After the addition of substrate (hydrogen peroxide), current decrease induced by its conversion by HRP has been detected by linear sweep voltammetry. Superparamagnetic microspheres were used for covalent immobilization of primary antibodies, which enables better antigen recognition compared to common sorption onto electrode surface.cze
dc.formatp. 347-356
dc.identifier.isbn978-80-7395-434-5 (printed)
dc.identifier.isbn978-80-7395-435-2 (on-line)
dc.identifier.urihttps://hdl.handle.net/10195/42518
dc.language.isoeng
dc.peerreviewedyes
dc.publicationstatuspublished
dc.publisherUniverzita Pardubicecze
dc.relation.ispartofSensing in electroanalysis. K. Kalcher, R. Metelka, I. Švancara, K. Vytřas (Eds.).2011, Volume 6.
dc.rightsopen access
dc.subjectvoltammetric detectioncze
dc.subjectbiosensorcze
dc.subjectovalbumincze
dc.subjectmagnetic microspherescze
dc.subjectscreen-printed electrodescze
dc.subjectELISAcze
dc.titleVoltammetric detection of ovalbumin at screen-printed electrodes in combination with immunomagnetic particlescze
dc.typeArticlecze
dspace.entity.typePublication

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6/2011 Sensing in Electroanalysis
Publikační činnost akademických pracovníků FChT / FChT Research Outputs