Determination of pesticide chlortoluron using HPLC with amperometric detection at a carbon paste electrode

Abstract

Method for the determination of pesticide chlortoluron in environmental samples was developed using HPLC with amperometric detection at the carbon paste electrode based on glassy carbon microparticles. Column LiChroCART® 125-4 Purospher® RP-18 (5 μm) was employed for the separation. Optimum conditions of the determination were mobile phase methanol and Britton-Robinson buffer pH 4.0 (60:40, v/v) and detection potential +1.3 V. Chlortoluron determination was performed in matrices of river water and soil; river water sample was filtered and injected directly; from 2.5 g of soil samples, the analyte was extracted to 5 mL of methanol. This method served well for the separation of the analyte from the matrix interferents and it was possible to determine chlortoluron content above the concentration 0.19 μmol L–1 in the case of river water and 0.29 μg g–1 in the case of soil.