Stability of enzymes immobilized in oriented way on magnetic and non-magnetic bead cellulose

Abstract

The enzymes galactose oxidase and neuraminidase have been immobilized in an oriented way to hydrazide derivative of magnetic and non-magnetic macroporous bead cellulose. Binding was accomplished after the sugar components have been oxidatively activated by natrium periodate as the oxidating agent. The operational and storage stability of neuraminidase reactor was studied during four weeks and operational stability of galactose oxidase reactor during three weeks. The operationas stability of galactose oxidase immobilized to magnetic macroporous bead cellulose was excellent in the environment of Cu 2+ ions. Stability of immobilized neuraminidase to non-magnetic support had a similar course. The stability of neuraminidase immobilized to magnetic macroporous bead cellulose was decreasing rapidly.