Rapid and simple preparation of thiol–ene emulsion-templated monoliths and their application as enzymatic microreactors
ČlánekOtevřený přístuppeer-reviewedpublishedDatum publikování
2015-03-27
Autoři
Lafleur, Josiane P.
Senkbeil, Silja
Novotný, Jakub
Nys, Gwenaël
Bøgelund, Nanna
Rand, Kasper D.
Foret, Frantisek
Kutter, Jörg P.
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Royal Society of Chemistry
Abstrakt
A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol–ene chemistry is presented. The method allows monolith synthesis and anchoring inside thiol–ene microchannels in a single photoinitiated step. Characterization by scanning electron microscopy showed that the methanol-based emulsion templating process resulted in a network of highly interconnected and regular thiol–ene beads anchored solidly inside thiol–ene microchannels. Surface area measurements indicate that the monoliths are macroporous, with no or little micro- or mesopores. As a demonstration, galactose oxidase and peptide-N-glycosidase F (PNGase F) were immobilized at the surface of the synthesized thiol–ene monoliths via two different mechanisms. First, cysteine groups on the protein surface were used for reversible covalent linkage to free thiol functional groups on the monoliths. Second, covalent linkage was achieved via free primary amino groups on the protein surface by means of thiol–ene click chemistry and L-ascorbic acid linkage. Thus prepared galactose oxidase and PNGase F microreactors demonstrated good enzymatic activity in a galactose assay and the deglycosilation of ribonuclease B, respectively.
Rozsah stran
p. 2162-2172
ISSN
1473-0197
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Lab on a Chip. 2015, vol. 15, issue 10
Vydavatelská verze
http://pubs.rsc.org/en/Content/ArticleLanding/2015/LC/C5LC00224A#!divAbstract
Přístup k e-verzi
open access
Attribution 3.0 Czech Republic
Attribution 3.0 Czech Republic
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
Přístup k tištěné verzi
Klíčová slova
Endorsement
Review
item.page.supplemented
item.page.referenced
Creative Commons license
Except where otherwised noted, this item's license is described as open access