Digitální knihovnaUPCE
 

Rapid and simple preparation of thiol–ene emulsion-templated monoliths and their application as enzymatic microreactors

ČlánekOtevřený přístuppeer-reviewedpublished
Náhled

Datum publikování

2015-03-27

Autoři

Lafleur, Josiane P.
Senkbeil, Silja
Novotný, Jakub
Nys, Gwenaël
Bøgelund, Nanna
Rand, Kasper D.
Foret, Frantisek
Kutter, Jörg P.

Vedoucí práce

Oponent

Název časopisu

Název svazku

Vydavatel

Royal Society of Chemistry

Abstrakt

A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol–ene chemistry is presented. The method allows monolith synthesis and anchoring inside thiol–ene microchannels in a single photoinitiated step. Characterization by scanning electron microscopy showed that the methanol-based emulsion templating process resulted in a network of highly interconnected and regular thiol–ene beads anchored solidly inside thiol–ene microchannels. Surface area measurements indicate that the monoliths are macroporous, with no or little micro- or mesopores. As a demonstration, galactose oxidase and peptide-N-glycosidase F (PNGase F) were immobilized at the surface of the synthesized thiol–ene monoliths via two different mechanisms. First, cysteine groups on the protein surface were used for reversible covalent linkage to free thiol functional groups on the monoliths. Second, covalent linkage was achieved via free primary amino groups on the protein surface by means of thiol–ene click chemistry and L-ascorbic acid linkage. Thus prepared galactose oxidase and PNGase F microreactors demonstrated good enzymatic activity in a galactose assay and the deglycosilation of ribonuclease B, respectively.

Rozsah stran

p. 2162-2172

ISSN

1473-0197

Trvalý odkaz na tento záznam

Projekt

Zdrojový dokument

Lab on a Chip. 2015, vol. 15, issue 10

Vydavatelská verze

http://pubs.rsc.org/en/Content/ArticleLanding/2015/LC/C5LC00224A#!divAbstract

Přístup k e-verzi

open access
Attribution 3.0 Czech Republic

Název akce

ISBN

Studijní obor

Studijní program

Signatura tištěné verze

Umístění tištěné verze

Přístup k tištěné verzi

Klíčová slova

Endorsement

Review

item.page.supplemented

item.page.referenced

Creative Commons license

Except where otherwised noted, this item's license is described as open access