A porcine model of skin wound infected with a polybacterial biofilm
ČlánekOmezený přístuppeer-reviewedpublishedDatum publikování
2018
Autoři
Klein, Pavel
Sojka, Martin
Kucera, Jan
Matonohova, Jana
Pavlik, Vojtech
Nemec, Jan
Kubickova, Gabriela
Slavkovsky, Rastislav
Szuszkiewicz, Katarzyna Maria
Danek, Petr
Vedoucí práce
Oponent
Název časopisu
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Vydavatel
Taylor & Francis Ltd.
Abstrakt
A clinically relevant porcine model of a biofilm-infected wound was established in 10 minipigs. The wounds of six experimental animals were infected with a modified polymicrobial Lubbock chronic wound biofilm consisting of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Bacillus subtilis. Four animals served as uninfected controls. The wounds were monitored until they had healed for 24 days. The biofilm persisted in the wounds up to day 14 and significantly affected healing. The control to infected healed wound area ratios were: 45%/21%, 66%/37%, and 90%/57% on days 7, 10 and 14, respectively. The implanted biofilm prolonged inflammation, increased necrosis, delayed granulation and impaired development of the extracellular matrix as seen in histological and gene expression analyses. This model provides a therapeutic one-week window for testing of anti-biofilm treatments and for research on the pathogenesis of wound infections in pig that is clinically the most relevant animal wound healing model.
Rozsah stran
p. 226-236
ISSN
0892-7014
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Projekt
Zdrojový dokument
Biofouling, volume 34, issue: 2
Vydavatelská verze
https://www.tandfonline.com/doi/abs/10.1080/08927014.2018.1425684
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pouze v rámci univerzity
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Klíčová slova
Wound, infection, biofilm, pig in vivo model, Poranění, Infekce, Biofilm, Prasečí in vivo model