Immunomagnetic sulfonated hypercrosslinked polystyrene microspheres forelectrochemical detection of proteins
ČlánekOmezený přístuppeer-reviewedpostprint Náhled není k dispozici
Soubory
Datum publikování
2011
Autoři
Šálek Petr
Korecká Lucie
Horák Daniel
Petrovský Eduard
Kovářová Jana
Metelka Radovan
Čadková Michaela
Bílková Zuzana
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Royal Society of Chemistry
Abstrakt
Poly(styrene-co-divinylbenzene) microspheres of narrow size distribution were prepared by (2-hydroxypropyl) cellulose-stabilized dispersion copolymerization of styrene and divinylbenzene in a 2-methoxyethanol/ethanol mixture under continuous addition of divinylbenzene. The copolymerization was initiated with dibenzoyl peroxide. The obtained microspheres were chloromethylated using several chloromethylation agents and then hypercrosslinked. Their porous structure was analyzed by nitrogen adsorption and mercury porosimetry. Superparamagnetic iron oxide nanoparticles were precipitated within the pores of microspheres from Fe(II) and Fe(III) chloride solution. The Fe content in the microspheres was determined by carbon analysis, atomic absorption spectroscopy and thermogravimetric analysis. Magnetic properties of the microspheres were characterized by magnetization curves and the temperature dependence of magnetic susceptibility. Finally, sulfo groups were introduced into the microspheres to prepare an immunomagnetic electrochemical biosensor for protein detection with ovalbumin as a model substance.
Rozsah stran
p. 14783-14792
ISSN
0959-9428
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Journal of Materials Chemistry, volume 21, issue: 38
Vydavatelská verze
Přístup k e-verzi
Pouze v rámci univerzity
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
Přístup k tištěné verzi
Klíčová slova
poly(glycidyl methacrylate) microspheres, dispersion polymerization, magnetic particles, miniemulsion polymerization, silica microspheres, size distribution, nanoparticles, beads, monodisperse, polyglycidyl methacrylátové mikročástice, disperzní polymerace, magnetické částice, proteiny, elektrochemická detekce