Zobrazit minimální záznam
dc.contributor.author |
Kopčil, Michal
|
|
dc.contributor.author |
Janeček, Radim
|
|
dc.contributor.author |
Kanďár, Roman
|
|
dc.contributor.editor |
Adam, Martin |
|
dc.date.accessioned |
2022-09-21T07:15:48Z |
|
dc.date.available |
2022-09-21T07:15:48Z |
|
dc.date.issued |
2022 |
|
dc.identifier.isbn |
978-80-7560-428-6 |
|
dc.identifier.issn |
1211-5541 |
|
dc.identifier.uri |
https://hdl.handle.net/10195/80395 |
|
dc.description.abstract |
A method has been developed to determine the levels of α-tocopherol in human plasma
and colon tissue using gas chromatography with mass spectrometric detection. The
samples were acquired from 27 patients with Crohn’s disease. Samples were
deproteinized and α-tocopherol was obtained using a two-step extraction and
measured by GC-MS without prior derivatization. The analytical parameters of the
method were satisfactory: the intra-day and inter-day coefficients of variation were
below 7% and quantitative recoveries range between 92.2-100.5%. The method shows
an excellent linear range from 0.6-64.3 μmol/L. The median measured α-tocopherol
concentration in human plasma was 21.6 μmol/L and in healthy colon tissue and tissue
affected by Crohn’s disease was 26.5 nmol/mg and 34.9 nmol/mg, respectively. We did
not find any statistically significant correlation or differences between the α-tocopherol
content in plasma and tissue. Overall, our method has potential to be easily implemented
in special studies for monitoring α-tocopherol as a biomarker of oxidative stress. |
en |
dc.format |
p. 51-59 |
|
dc.language.iso |
eng |
|
dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 28/2022 |
en |
dc.rights |
open access |
en |
dc.subject |
α-Tocopherol |
en |
dc.subject |
Crohn’s disease |
en |
dc.subject |
colon tissue |
en |
dc.subject |
GC-MS |
en |
dc.title |
Determination of α-tocopherol in different biological samples by GC-MS without derivatization |
en |
dc.type |
article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
dc.identifier.obd |
39887569 |
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