Determination of α-tocopherol in different biological samples by GC-MS without derivatization

Show simple item record Kopčil, Michal Janeček, Radim Kanďár, Roman
dc.contributor.editor Adam, Martin 2022-09-21T07:15:48Z 2022-09-21T07:15:48Z 2022
dc.identifier.isbn 978-80-7560-428-6
dc.identifier.issn 1211-5541
dc.description.abstract A method has been developed to determine the levels of α-tocopherol in human plasma and colon tissue using gas chromatography with mass spectrometric detection. The samples were acquired from 27 patients with Crohn’s disease. Samples were deproteinized and α-tocopherol was obtained using a two-step extraction and measured by GC-MS without prior derivatization. The analytical parameters of the method were satisfactory: the intra-day and inter-day coefficients of variation were below 7% and quantitative recoveries range between 92.2-100.5%. The method shows an excellent linear range from 0.6-64.3 μmol/L. The median measured α-tocopherol concentration in human plasma was 21.6 μmol/L and in healthy colon tissue and tissue affected by Crohn’s disease was 26.5 nmol/mg and 34.9 nmol/mg, respectively. We did not find any statistically significant correlation or differences between the α-tocopherol content in plasma and tissue. Overall, our method has potential to be easily implemented in special studies for monitoring α-tocopherol as a biomarker of oxidative stress. en
dc.format p. 51-59
dc.language.iso eng
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 28/2022 en
dc.rights open access en
dc.subject α-Tocopherol en
dc.subject Crohn’s disease en
dc.subject colon tissue en
dc.subject GC-MS en
dc.title Determination of α-tocopherol in different biological samples by GC-MS without derivatization en
dc.type article en
dc.peerreviewed yes en
dc.publicationstatus published en

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