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dc.contributor.author |
Štramová, Xenie
|
|
dc.contributor.author |
Drábková, Petra
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dc.contributor.author |
Kanďár, Roman
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dc.date.accessioned |
2020-06-10T08:40:03Z |
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dc.date.available |
2020-06-10T08:40:03Z |
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dc.date.issued |
2013 |
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dc.identifier.isbn |
978-80-7395-748-3 |
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dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
https://hdl.handle.net/10195/75464 |
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dc.description.abstract |
An ion-exchange high-performance liquid chromatography with ultraviolet
detection for the determination of creatine in human serum has been developed.
The human serum was deproteinized with perchloric acid. For the separation, a
cation-exchange column LC-SCX, 250 mm × 4.6 mm, 5 μm, was used. The mixture
of ethanol and 10 mmol l–1 ammonium acetate (15:85, v/v), pH 4.00 ± 0.05 was
used as a mobile phase. The analytical performance of this method is satisfactory:
the intra-assay and inter-assay coefficients of variation were below 10 %.
Quantitative recoveries of spiked serum samples were between 97.1 % and 103.5
%. The calibration curve was linear in the whole range tested. The limit of
quantification was 2 μmol l–1. The preliminary reference ranges of serum creatine
in a group of blood donors are 17-89 μmol l–1 for women and 21-97 μmol l–1 for
men. |
en |
dc.format |
p. 39–48 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19/2013 |
en |
dc.rights |
open access |
en |
dc.title |
An assay of creatine in human serum using an HPLC with UV detection |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
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