Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot

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dc.contributor.author Kučerová, Jana
dc.contributor.author Holubová, Lucie
dc.contributor.author Jankovičová, Barbora
dc.contributor.author Dvořáková, Veronika
dc.contributor.author Moťková, Petra
dc.contributor.author Bílková, Zuzana
dc.date.accessioned 2020-06-10T08:14:07Z
dc.date.available 2020-06-10T08:14:07Z
dc.date.issued 2013
dc.identifier.isbn 978-80-7395-748-3
dc.identifier.issn 1211-5541
dc.identifier.uri https://hdl.handle.net/10195/75462
dc.description.abstract The selection of antibodies with the desired specificity, immunoreactivity, purity and integrity is a key step in developing an effective immunosorbent for immunomagnetic separation (IMS). The choice of methods for characterizing the antibodies is limited, especially with antibodies specific to surface cell structures. In this study, four commercial anti-Salmonella antibodies were evaluated from the point of view of their immunoreactivity with the cells of Salmonella Typhimurium and of their purity. For these purposes, traditional SDS-PAGE analysis with subsequent silver staining and a newly adapted whole-cell dot blot technique were applied. Based on this testing, monoclonal anti-core LPS Salmonella antibodies from MyBiosource unambiguously demonstrated the highest immunoreactivity to Salmonella Typhimurium cells, whereas cross-reactivity with the closely related G- bacteria, Escherichia coli and Citrobacter braakii was not observed. Such antibodies will be subsequently used for the preparation of an anti-Salmonella immunosorbent and applied in IMS. The immunoreactivity and selectivity of anti- Salmonella poly- and monoclonal antibodies are also discussed. en
dc.format p. 57–66
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19/2013 en
dc.rights open access en
dc.title Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en

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