Zdrojový dokument:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 19/2013
ISSN:1211-5541
Abstrakt:
The selection of antibodies with the desired specificity, immunoreactivity, purity
and integrity is a key step in developing an effective immunosorbent for
immunomagnetic separation (IMS). The choice of methods for characterizing the
antibodies is limited, especially with antibodies specific to surface cell structures.
In this study, four commercial anti-Salmonella antibodies were evaluated from the
point of view of their immunoreactivity with the cells of Salmonella Typhimurium
and of their purity. For these purposes, traditional SDS-PAGE analysis with
subsequent silver staining and a newly adapted whole-cell dot blot technique were
applied. Based on this testing, monoclonal anti-core LPS Salmonella antibodies
from MyBiosource unambiguously demonstrated the highest immunoreactivity to
Salmonella Typhimurium cells, whereas cross-reactivity with the closely related G- bacteria, Escherichia coli and Citrobacter braakii was not observed. Such
antibodies will be subsequently used for the preparation of an anti-Salmonella
immunosorbent and applied in IMS. The immunoreactivity and selectivity of anti-
Salmonella poly- and monoclonal antibodies are also discussed.