Show simple item record
dc.contributor.author |
Soukup, Jan
|
|
dc.contributor.author |
Janás, Petr
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dc.contributor.author |
Jandera, Pavel
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dc.date.accessioned |
2020-05-20T09:15:24Z |
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dc.date.available |
2020-05-20T09:15:24Z |
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dc.date.issued |
2014 |
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dc.identifier.isbn |
978-80-7395-814-5 |
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dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
https://hdl.handle.net/10195/75432 |
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dc.description.abstract |
We studied the influence of mobile phase composition on the retention of phenolic
acids and flavonoid compounds on five different hydrosilated silica-based
stationary phases in buffered aqueous acetonitrile. Cogent UDC cholesterolTM,
Cogent bidentate C18TM and Cogent Phenyl hydrideTM columns show significant
dual reversed-phase/normal-phase retention behaviour, while Cogent Diamond
hydrideTM and Cogent Silica-CTM columns provide very low retention in the
reversed-phase mode. The effect of the aqueous acetate buffer concentration on
retention factors of phenolic acids and flavonoid compounds over the full mobile
phase composition range, including both aqueous normal-phase (ANP) and
reversed-phase (RP) mechanisms, can be described by a four-parameter equation
for dual-retention mechanism. At increasing temperature, the retention factors
and peak widths decrease in the aqueous normal-phase range. In agreement with
van’t Hoff model, linear ln k versus 1/T plots were observed, showing a single retention mechanism. From among the stationary phases tested, Cogent UDC
cholesterolTM column has high temperature stability (up to 100 °C) and provides
the most selective and efficient separations of flavones in the ANP mode and
Cogent Diamond hydrideTM is the most selective and effective for separation of
phenolic acids. |
en |
dc.format |
p. 37–70 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 20/2014 |
en |
dc.rights |
open access |
en |
dc.title |
Utilization of hydrosilated silica-based stationary phases for separation of antioxidants |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
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