Zobrazit minimální záznam
dc.contributor.author |
Chagovets, Vitaliy
|
|
dc.contributor.author |
Holčapek, Michal
|
|
dc.contributor.author |
Lísa, Miroslav
|
|
dc.date.accessioned |
2020-05-20T08:55:48Z |
|
dc.date.available |
2020-05-20T08:55:48Z |
|
dc.date.issued |
2014 |
|
dc.identifier.isbn |
978-80-7395-814-5 |
|
dc.identifier.issn |
1211-5541 |
|
dc.identifier.uri |
https://hdl.handle.net/10195/75430 |
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dc.description.abstract |
This work presents a computational method for deconvolution of interfered peaks
of protonated and sodiated phosphatidylcholines (PCs) to solve the problem of
relative quantitation of these PCs. The method is based on the fact that adducts
of PCs with proton and sodium ion give unique characteristic peaks in tandem
mass spectra. The intensities of these peaks were used to find out contribution of
protonated and sodiated PCs to the formation of interfered peak in full-scan mass
spectrum and, further, to correlate peak intensities that correspond to PCs in fullscan
mass spectrum with PC’s concentrations. The model was calibrated on
synthesized PCs and verified for applicability in the range of Na+ ion
concentrations from trace to physiological one. |
en |
dc.format |
p. 111–121 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 20/2014 |
en |
dc.rights |
open access |
en |
dc.title |
Relative quantitation of phosphatidylcholines with interfered masses of protonated and sodiated molecules by matrix-assisted laser desorption/ionization mass spectroscopy |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
published |
en |
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