An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high-performance liquid chromatography with fluorescence detection

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dc.contributor.author Drábková, Petra
dc.contributor.author Martínková, Jiřina
dc.contributor.author Míková, Veronika
dc.contributor.author Štramová, Xenie
dc.contributor.author Kanďár, Roman
dc.date.accessioned 2020-05-13T18:59:42Z
dc.date.available 2020-05-13T18:59:42Z
dc.date.issued 2012
dc.identifier.isbn 978-80-7395-577-9
dc.identifier.issn 1211-5541
dc.identifier.uri https://hdl.handle.net/10195/75383
dc.description.abstract A method is described for the determination of 4-hydroxy-trans-2-nonenal in human seminal plasma. Semen samples were obtained from male partners of couples presenting for a fertility evaluation. After liquefaction, the samples were centrifuged and the seminal plasma was stored at –80 °C. 4-Hydroxy-trans-2- nonenal was derivatized with 1,3-cyclohexanedione to generate the fluorescent alkyl acridine derivative. After derivatization, seminal plasma proteins were precipitated with cold perchloric acid, and the supernatant was injected into the HPLC system. The separation was realized on an analytical reversed-phase column with fluorescence detection. The mixture of ethanol and deionized water was used as the mobile phase. The intra-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked seminal plasma were between 62.0 and 81.0 %. en
dc.format p. 17–28
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 18/2012 en
dc.rights open access en
dc.title An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high-performance liquid chromatography with fluorescence detection en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en


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