Zdrojový dokument:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 18/2012
ISSN:1211-5541
Abstrakt:
A method is described for the determination of 4-hydroxy-trans-2-nonenal in
human seminal plasma. Semen samples were obtained from male partners of
couples presenting for a fertility evaluation. After liquefaction, the samples were
centrifuged and the seminal plasma was stored at –80 °C. 4-Hydroxy-trans-2-
nonenal was derivatized with 1,3-cyclohexanedione to generate the fluorescent
alkyl acridine derivative. After derivatization, seminal plasma proteins were
precipitated with cold perchloric acid, and the supernatant was injected into the
HPLC system. The separation was realized on an analytical reversed-phase
column with fluorescence detection. The mixture of ethanol and deionized water
was used as the mobile phase. The intra-assay coefficients of variation were below
10 %. Quantitative recoveries from spiked seminal plasma were between 62.0 and
81.0 %.