Secondary reaction of amino acid L-proline with 4-acetamido-o-benzoquinone as
a product of the enzymatic oxidation of acetaminophen mediated by mushroom
tyrosinase isolated from Agaricus bisporus has been studied by amperometric
detection in the batch injection analysis configuration and by ultraviolet-visible
spectrophotometry. Actually, this scientific work is focused on finding out whether
it would be possible to develop an indirect electrochemical method based on the
amperometric biosensing suitable for monitoring of the L-proline in various honey
samples. For optimum content of acetaminophen (100 :mol dm–3) and tyrosinase
(3.33 :g cm–3), a satisfactory linear range from 0.1 to 0.5 mmol dm–3 L-proline was
obtained for absorption maximum at 332 nm. However, it is necessary to mention
that the indirect determination of L-proline by the amperometric tyrosinase
biosensor still presents a challenge in the field of developing biological sensors for
monitoring of this amino acid.
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dc.format
p. 85–97
dc.language.iso
en
dc.publisher
University of Pardubice
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dc.relation.ispartof
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 23/2017
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dc.rights
open access
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dc.title
Catalitic activity of Agaricus bisporus mushroom tyrosinase to acetaminophenon in the presence of L-proline