This work is focused on the determination of proline in 24 Czech mead samples using
reversed-phase high performance liquid chromatography (RP-HPLC) coupled with
fluorescence detector (operating at excitation wavelength λex = 264 nm and emission
wavelength λem = 497 nm). Sample pre-treatment including solid phase extraction of
mead by Strata SCX SPE column and a derivatization of the extract acquired by dansyl
chloride was performed and thoroughly optimised. Derivative of proline was separated
using Ascentis Express C18 column (150 × 3 mm, 2.7 μm) in the combination with binary
mobile phases that had consisted of 0.1% TEA (pH 3.0) and 100% acetonitrile. The
method proposed could be used for evaluation of mead quality and its authenticity.
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dc.format
p. 15 - 26
dc.language.iso
en
dc.publisher
University of Pardubice
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dc.relation.ispartof
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 25/2019
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dc.rights
open access
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dc.subject
proline
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dc.subject
dansyl chloride
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dc.subject
meads
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dc.subject
derivatization
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dc.subject
extraction
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dc.subject
liquid chromatography
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dc.title
Determination of proline in meads using high performance liquid chromatography