Development of the magnetic bioaffinity carrier for the anti-amyloid beta 1–42 antibodies detection

Show simple item record Slováková, Marcela Murasová, Pavla Vozandychová, Věra Palarčík, Jiří Podzemná, Veronika Bílková, Zuzana 2019-10-03T10:51:53Z 2019-10-03T10:51:53Z 2019
dc.identifier.isbn 978-80-7560-243-5
dc.identifier.issn 1211-5541
dc.description.abstract In this paper, the development of a new affinity carrier was the key point for the anti-amyloid ß 1–42 antibodies detection in human serum, as the potential marker of the Alzheimer’s disease. Magnetic solid-phase preparation comprised the amyloid ß 1–42 peptide immobilization on the superparamagnetic carboxyl beads that had been modified by bisamino-polyethylene glycol spacer to gain terminal amine groups and subsequently analyzed by the zeta potential measurement. The following immobilization of the amyloid ß 1–42 peptide onto the modified superparamagnetic beads was performed by the carbodiimide chemistry. Nonspecific sorption of serum contaminants was prevented by using of bovine serum albumin. The developed magnetic carrier was analyzed by scanning electron microscopy. The amyloid ß 1–42 peptide bioaffinity carrier was applied for detection of natural anti-amyloid ß 1–42 antibodies in human serum. A comparison with spacer-free bioaffinity carrier has confirmed a divergent optical density values for control serum sample. The new bioaffinity carrier was applied to the detection of anti-amyloid ß 1–42 antibodies in control serum and in sera of patients with neurodegenerative disorders. en
dc.format p. 41 - 52
dc.language.iso en
dc.publisher University of Pardubice en
dc.relation.ispartof Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 25/2019 en
dc.rights open access en
dc.subject anti-amyloid ß 1–42 en
dc.subject magnetic bioaffinity carrier en
dc.subject immobilization en
dc.title Development of the magnetic bioaffinity carrier for the anti-amyloid beta 1–42 antibodies detection en
dc.type Article en
dc.peerreviewed yes en
dc.publicationstatus published en
dc.identifier.obd 39882829

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