Zdrojový dokument:Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 24/2018
ISSN:1211-5541
Abstrakt:
Adsorptive stripping voltammetry (AdSV) of cholecalciferol at the glassy carbon
electrode (GCE) was developed and based on existing conventional approaches
employing anodic oxidation in pure organic electrolytes or aqueous-organic mixtures.
Despite a rather poor reproducibility of the adsorption-driven process (with the RSD of
ca. 10 %), significantly higher sensitivity was achieved. As optimum conditions, the
respective procedure comprised an accumulation of cholecalciferol from 40% ethanol
at 300 min–1 for 8 min followed by electrochemical detection in the Britton-Robinson
buffer (pH 4.0) using square-wave voltammetry at a potential step of 5 mV, amplitude
of 35 mV, and frequency of 40 Hz