Comparative cytotoxicity of chelidonine and homochelidonine, the dimethoxy analogues isolated from Chelidonium majus L. (Papaveraceae), against human leukemic and lung carcinoma cells

Show simple item record Havelek, Radim cze Seifrtova, Martina cze Královec, Karel cze Kročová, Eliška cze Tejkalová, Veronika cze Novotny, Ivan cze Cahlikova, Lucie cze Safratova, Marcela cze Opletal, Lubomir cze Bílková, Zuzana cze Vavrova, Jirina cze Rezacova, Martina cze 2017-05-11T11:07:43Z 2017-05-11T11:07:43Z 2016 eng
dc.identifier.issn 0944-7113 eng
dc.description.abstract In this study the effects of naturally occurring homochelidonine in comparison to chelidonine on cell cycle progression and cell death in leukemic T-cells with different p53 status are described. We found that homochelidonine and chelidonine displayed significant cytotoxicity in examined blood cancer cells with the exception of HEL 92.1.7 and U-937 exposed to homochelidonine. Unexpectedly, homochelidonine and chelidonine-induced cytotoxicity was more pronounced in Jurkat cells contrary to MOLT-4 cells. Homochelidonine showed an antiproliferative effect on A549 cells but it was less effective compared to chelidonine. Biphasic dose-depended G1 and G2/M cell cycle arrest along with the population of sub-G1 was found after treatment with homochelidonine in MOLT-4 cells. In variance thereto, an increase in G2/M cells was detected after treatment with homochelidonine in Jurkat cells. Treatment with chelidonine induced cell cycle arrest in the G2/M cell cycle in both MOLT-4 and Jurkat cells. MOLT-4 and Jurkat cells treated with homochelidonine and chelidonine showed features of apoptosis such as phosphatidylserine exposure, a loss of mitochondrial membrane potential and an increase in the caspases -3/7, -8 and -9. Western blots indicate that homochelidonine and chelidonine exposure activates Chk1 and Chk2. Studies conducted with fluorescence microscopy demonstrated that chelidonine and homochelidonine inhibit tubulin polymerization in A549 cells. Collectively, the data indicate that chelidonine and homochelidonine are potent inducers of cell death in cancer cell lines, highlighting their potential relevance in leukemic cells. eng
dc.format p. 253-266 eng
dc.language.iso eng eng
dc.publisher Elsevier GmbH eng
dc.relation.ispartof Phytomedicine, volume 23, issue: 3 eng
dc.rights práce není přístupná eng
dc.subject Homochelidonine eng
dc.subject Apoptosis eng
dc.subject Biphasic cell cycle arrest eng
dc.subject Mitotic block eng
dc.subject Homochelidonin cze
dc.subject Apoptóza cze
dc.subject Bifázická zástava buněčného cyklu cze
dc.subject Mitotický blok cze
dc.title Comparative cytotoxicity of chelidonine and homochelidonine, the dimethoxy analogues isolated from Chelidonium majus L. (Papaveraceae), against human leukemic and lung carcinoma cells eng
dc.title.alternative Porovnání cytotoxicity chelidoninu a homochelidoninu, dimethoxy analoga izolovaného z Chelidonium majus L. (Papaveraceae), vůči lidským leukemickým a plicním nádorovým buňkám cze
dc.type article eng
dc.description.abstract-translated Tato práce porovnává cytotoxicitu chelidoninu a homochelidoninu vůči lidským leukemickým a plicním nádorovým buňkám. Výsledky studie naznačují, že chelidonin a homochelidonin jsou účinnými aktivátory apoptózy u nádorových buněčných linií. cze
dc.peerreviewed yes eng
dc.publicationstatus published version eng
dc.identifier.doi 10.1016/j.phymed.2016.01.001 eng
dc.identifier.wos 000371781500003 eng
dc.identifier.scopus 2-s2.0-84977621316
dc.identifier.obd 39877075 eng

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