dc.contributor.author |
Vorčáková Katarína
|
|
dc.contributor.author |
Štěpánková Šárka
|
|
dc.contributor.author |
Sedlák Miloš
|
|
dc.contributor.author |
Vytřas Karel
|
|
dc.date.accessioned |
2016-11-14T08:20:07Z |
|
dc.date.available |
2016-11-14T08:20:07Z |
|
dc.date.issued |
2015 |
|
dc.identifier.issn |
2227-9040 |
|
dc.identifier.uri |
http://hdl.handle.net/10195/66570 |
|
dc.description.abstract |
The inhibitory effect of nine phenylcarbamates with various substituents was studied. For this purpose, electrochemical sensors were applied under two different conditions: if an enzyme was present in the solution or if the enzyme was immobilized onto the electrode surface. In both cases, 3-[(ethoxycarbonyl)amino]phenyl (4-chloro-phenyl)carbamate was found as the most effective inhibitor for butyrylcholinesterase. The best inhibitors for acetylcholinesterase were determined as well, depending on the used method. Thus, 3-[(butoxycarbonyl)-amino]phenyl phenylcarbamate with the enzyme present in the solution and 3-[(ethoxycarbonyl)amino]phenyl (3-methylphenyl)carbamate when the enzyme was immobilized onto the electrode surface were evaluated as the most effective inhibitors. Michaelis constants as well as maximum reaction rates were calculated and assessed. |
eng |
dc.format |
p. 274-283 |
eng |
dc.language.iso |
eng |
|
dc.relation.ispartof |
Chemosensors, volume 3, issue: 4 |
eng |
dc.rights |
Práce není přístupná |
eng |
dc.subject |
biosensors |
eng |
dc.subject |
immobilization of cholinesterases |
eng |
dc.subject |
phenylcarbamates |
eng |
dc.subject |
inhibition |
eng |
dc.subject |
IC50 |
eng |
dc.subject |
kinetic parameters |
eng |
dc.subject |
biosenzory |
cze |
dc.subject |
imobilizace cholinesteras |
cze |
dc.subject |
fenylkarbamaty |
cze |
dc.subject |
inhibice |
cze |
dc.subject |
IC50 |
cze |
dc.subject |
kinetické parametry |
cze |
dc.title |
Electrochemical Sensors for the Estimation of the Inhibitory Effect of Phenylcarbamates to Cholinesterase |
eng |
dc.title.alternative |
Elektrochemické senzory pro stanovení inhibičního účinku fenylkarbamatů na cholinesterázy |
cze |
dc.type |
article |
eng |
dc.description.abstract-translated |
U devíti látek ze skupiny fenylkarbamátů byla sledovaná inhibiční účinnost na cholinesterázy. V druhém kroku byly stanoveny kinetické parametry. Byly použité elektrochemické senzory za použití 2 podmínek, enzym v roztoku a enzym imobilizovaný. Následně byly vyhodnocené nejúčinnější látky a diskutován vliv imobilizace na kinetické parametry. |
cze |
dc.peerreviewed |
yes |
eng |
dc.publicationstatus |
postprint |
eng |
dc.identifier.wos |
000367617800003 |
|
dc.identifier.obd |
39874794 |
|