Zdrojový dokument:Sensing in Electroanalysis. (K. Kalcher, R. Metelka, I. Švancara, K. Vytřas; Eds.). 2013/2014, Volume 8.
Abstrakt:
In this paper, pretreated pencil graphite electrode (Pre-PGE) was used for the determination of insulin by anodic stripping differential pulse voltammetry (DPASV). The
influences of the pretreatment procedures, pH values, accumulation potential and time on the
signal enhancement of insulin were optimized, and a novel electrochemical method for the determination of insulin was described. The currents obtained from DPASV measurements at
optimum conditions (phosphate buffer solution pH 7.0), accumulation time (60 seconds), applied potential 400 mV vs. Ag/AgCl/3.0 mol L−1 KCl) were linearly correlated with the concentration of insulin. Calibration curve was obtained for insulin concentrations in the range of 5.0-200.0×10-9 mol L−1 .The limit of detection was found to be 2.0×10-9 mol L−1.