Performance evaluation of acetylcholinesterase-based biosensors for detection of heavy metals

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dc.contributor.author David, Melinda
dc.contributor.author Badea, Mihaela
dc.contributor.author Florescu, Monica
dc.date.accessioned 2012-03-07T07:52:51Z
dc.date.available 2012-03-07T07:52:51Z
dc.date.issued 2011
dc.identifier.isbn 978-80-7395-434-5 (printed)
dc.identifier.isbn 978-80-7395-435-2 (on-line)
dc.identifier.uri http://hdl.handle.net/10195/42520
dc.description.abstract In this work, mediated carbon-ink screen-printed electrodes are used to develop enzyme-based biosensors for detection of selected heavy metals. Two immobilisation methods were used for acetylcholinesterase (AChE) immobilisation on electrode surface together with two redox mediators. The methods used for the encapsulation of the enzyme were: cross-linking with bi-functional reagents like glutar-aldehyde (GA) and encapsulation with sol-gel method.The used mediators were TCNQ (tetracianoquinodimethan) and CoPC (cobalt phthalocyanine). Comparison between biosensors performance was made function of electrolyte pH, especially for more acidic values were heavy metals are present in ionic form. cze
dc.format p. 337-346
dc.language.iso eng
dc.publisher Univerzita Pardubice cze
dc.relation.ispartof Sensing in electroanalysis. K. Kalcher, R. Metelka, I. Švancara, K. Vytřas (Eds.).2011, Volume 6.
dc.rights open access
dc.subject biosensors cze
dc.subject screen-printed electrodes cze
dc.subject acetylcholinesterase cze
dc.subject heavy metals cze
dc.title Performance evaluation of acetylcholinesterase-based biosensors for detection of heavy metals cze
dc.type Article cze
dc.peerreviewed yes
dc.publicationstatus published


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