Zdrojový dokument:Sensing in electroanalysis. K. Vytřas, K. Kalcher, I. Švancara (Eds.). 2010, Volume 5.
Abstrakt:
A new method for the determination of selected aminobiphenyls (ABs)
and aminonaphthalenes (ANs), proved or suspected carcinogens and mutagens, was
developed using reversed-phase HPLC coupled to an amperometric platinum tubular
detector and a spectrophotometric detector. Using mobile phase consisting of
acetonitrile and phosphate buffer pH 2.5 (40:60, v/v), separation of 2- and
4-aminobiphenyl and 1- and 2-aminonaphthalene was achieved within 10 min and
limits of determination between 3·10–8 mol L–1 and 8·10–8 mol L–1 were obtained for
amperometric detection. In comparison with spectrophotometric detection, it is
superior in both sensitivity and selectivity. The developed method was applied on the
analysis of spiked samples of urine. Solid phase extraction was used for a preliminary
separation and preconcentration of the tested analytes, leading to the decrease of
limits of determination to the values between 7.2·10–9 mol L–1 and 2.7·10–8 mol L–1.
