| dc.contributor.advisor |
Bílková, Zuzana |
cze |
| dc.contributor.author |
Pavlasová, Petra
|
|
| dc.date.accessioned |
2010-07-03T11:36:44Z |
|
| dc.date.available |
2010-07-03T11:36:44Z |
|
| dc.date.issued |
2010 |
|
| dc.identifier |
Univerzitní knihovna (sklad) |
cze |
| dc.identifier.uri |
http://hdl.handle.net/10195/36327 |
|
| dc.description.abstract |
Mezi faktory virulence řady patogenních bakterií patří mimo jiné sekretované proteiny. Sekretované proteiny jsou transportovány skrze bakteriální membrány různými mechanismy, prostřednictvím různých sekrečních systémů. Doposud bylo odhaleno a popsáno šest různých sekrečních systémů bakterií. Uvolňování membránových vesiklů je rovněž způsob, kterým bakterie mohou transportovat proteiny do extracelulárního prostředí. Na základě této skutečnosti je uvolňování membránových vesiklů považováno rovněž některými autory za sekreční systém bakterií. Při sekreci skrze membránové vesikly dochází k uvolňování "váčků" vycházejících z bakteriální membrány, které mohou být naplněny různými komponentami jako jsou: proteiny, |
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| dc.format |
87 s. |
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| dc.format.extent |
2100912 bytes |
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| dc.format.mimetype |
application/pdf |
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| dc.language.iso |
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|
| dc.publisher |
Univerzita Pardubice |
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| dc.rights |
Práce není přístupná |
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| dc.subject |
F. tularensis |
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| dc.subject |
Chamberlainovo médium |
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| dc.subject |
vesikly vnější membrány |
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| dc.subject |
Stirred cell 8200 |
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| dc.subject |
peleta |
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| dc.subject |
F. tularensis |
eng |
| dc.subject |
Chamberlain cultivation medium |
eng |
| dc.subject |
outer membrane vesicles |
eng |
| dc.subject |
Stirred cell 8200 |
eng |
| dc.subject |
pelet |
eng |
| dc.title |
Membránové vesikly bakterie F. tularensis |
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| dc.title.alternative |
The membrane vesicles of bacterium F. tularensis |
eng |
| dc.type |
diplomová práce |
cze |
| dc.date.accepted |
2010 |
cze |
| dc.description.abstract-translated |
Secreted proteins belong to virulence factors of many pathogenic bacteria. Several distinct mechanisms are used to transport secreted proteins via bacterial membranes, using various secreted systems. So far, six different bacterial secretion systems were discovered and described. Bacteria can transport proteins to extracellular matrix space via releasing membrane vesicles. Based on this phenomenon, some authors view the process of membrane vesicles release as the secretion system of bacteria. The bacteria membrane releases blebs which can contain various components including proteins, toxins, DNA, antimicrobials, antibiotic resistance determinants and others. Pathogenetic mechanism of disease induced by bacterium Francisella tularensis (F. tularensis) has not been completely described yet. My thesis is focused on studying the membrane vesicles of bacterium F. tularensis. The primary aim of this study was to prove whether gram-negative bacterium F. tularensis, likewise other gram-negative bacteria, is able of secretion via the membrane vesicles. The experimental part was focused on optimisation of the methodology for the puprose of detection or isolation the ofmembrane vesicles. The protein cargo cargo of the F. tularensis membrane vesicles will be studied in the future. Several strains of bacterium F. tularensis were cultivated under different conditions (in complete Chamberlain cultivation medium, in cultivation medium without Fe, in cultivation medium with hydrogen peroxide as an added "stress factor") untill the late log growth phase. After cultivation the bacteria filtrates were obtained and concentrated by Stirred Cell 8200 concentration device (made by Millipore Corp.). The next important task was to find appropriate conditions of ultracentrifugation which would lead to obtaining the pelet, containing the membrane vesicles. The samples were purified by gradient separation method so that the proteomic analysis of vesicules content. The presence of the membrane vesicles in the analysed samples was visualized by electron microscopy in co-operation with Dr. Benada (Institute of Microbiology, Academy of Sciences of the Czech Republic). It was concluded that the optimisation of the procedures leading to obtaining the membrane vesicles was succesfully accomplished and the membrane vesicles were indeed detected by electron microscopy. The protein cargo of the membrane vesicles will be studied further in the future. This topic falls outside my thesis. |
eng |
| dc.description.department |
Katedra biologických a biochemických věd |
cze |
| dc.thesis.degree-discipline |
Analýza biologických materiálů |
cze |
| dc.thesis.degree-name |
Mgr. |
cze |
| dc.thesis.degree-grantor |
Univerzita Pardubice. Fakulta chemicko-technologická |
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| dc.identifier.signature |
D21803 |
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| dc.identifier.signature |
D21803 |
|
| dc.thesis.degree-program |
Speciální chemicko-biologické obory |
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| dc.description.defence |
1. Prezentace výsledků diplomové práce. 2. Diskuze k posudkům vedoucího a oponenta diplomové práce. 3. Diplomantka zodpověděla všechny dotazy a připomínky k DP. |
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| dc.description.grade |
Dokončená práce s úspěšnou obhajobou |
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