(University of Pardubice, 2020) Pravcová, Kateřina; Mikysek, Tomáš; Česlová, Lenka
The content of 5-hydroxymethylfurfural (HMF) in honey and mead is strongly
influenced by the technological process of these products and could be used as the
parameter of choice to assess the quality of honey and mead. Therefore, the aim of this
work was the comparison of well established reversed-phase high performance liquid
chromatography (RP-HPLC) with voltammetric determination at the glassy carbon
electrode. The final HPLC separation was performed on an Ascentis Express C18
column (150 × 3 mm, 2.7 μm) using isocratic elution with 10 % methanol in water.
In total, 9 samples (6 honeys and 3 meads) were used for determination of the content
of HMF.