Digitální knihovnaUPCE
 

26 (2020) Scientific papers, Series A

Permanentní URI k tomuto záznamuhttps://hdl.handle.net/10195/76706

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20202

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Typ: search.filters.itemtype.ArticleAutor: search.filters.author.Mikysek, Tomáš

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  • Náhled
    Článekpeer-reviewedpublishedOtevřený přístup
    Comparison of two different approaches for determination of biomimetic activity of selected copper complexes
    (University of Pardubice, 2020) Sýs, Milan; Novák, Miroslav; Mikysek, Tomáš
    In this article, an evaluation of the catecholase activity of the recently synthesized copper complexes towards dopamine is reported based on a comparison of the performance of two different techniques – UV-Vis spectrophotometry and hydrodynamic amperometry. The results obtained were confronted with those published for natural tyrosinase enzyme (isolated from mushroom 'Agaricus bisporus') and defined by Michaelis–Menten kinetics model. Besides a finding of comparable catalytic activity for the copper catalysts tested, it has to be emphasised that all the measurements have been performed in methanol, in which tyrosinase enzyme loses its catalytic activity.
  • Náhled
    Článekpeer-reviewedpublishedOtevřený přístup
    Comparison of HPLC and electrochemical determination of 5-hydroxymethylfurfural in honey and mead samples
    (University of Pardubice, 2020) Pravcová, Kateřina; Mikysek, Tomáš; Česlová, Lenka
    The content of 5-hydroxymethylfurfural (HMF) in honey and mead is strongly influenced by the technological process of these products and could be used as the parameter of choice to assess the quality of honey and mead. Therefore, the aim of this work was the comparison of well established reversed-phase high performance liquid chromatography (RP-HPLC) with voltammetric determination at the glassy carbon electrode. The final HPLC separation was performed on an Ascentis Express C18 column (150 × 3 mm, 2.7 μm) using isocratic elution with 10 % methanol in water. In total, 9 samples (6 honeys and 3 meads) were used for determination of the content of HMF.