27 (2021) Scientific papers, Series A
Permanentní URI k tomuto záznamuhttps://hdl.handle.net/10195/78444
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Článekpeer-reviewedpublished Otevřený přístup Biogenic amines in wine and cheese(University of Pardubice, 2021) Švecová, Blanka; Janovská, Miroslava; Adam, MartinBiogenic amines in ten samples of wine and five samples of cheese were determined using an HPLC-UV-MS method. Inevitable derivatization was performed with the reagent dansyl chloride, and the derivatives prepared were analysed under optimized chromatographic conditions in reversed phase mode. An octadecylsilicagel column (C18) was used for the separation of ten selected biogenic amines derivatives which were eluted using the mobile phase composed of 5 mM ammonium formate and acetonitrile at a flow-rate 0.6 mL min−1 and detected at the wavelength 254 nm. Basic validation of this method was carried out as well. Limits of detection and quantitation, external standard calibration, intra-day repeatability and inter-day reproducibility for spectrophotometric detection were determined. Five various red wines, four white wines and one rosé wine contained very small amounts of biogenic amines. The highest total content was approximately 2.5 mg L−1, which represents a relatively low content in comparison with some results published in literature. Similarly, the content of biogenic amines in cheeses analysed was lower than expected, even in case of fermented cheeses. The highest content was found in one of the typical Czech fermented cheeses called Romadur and being 3.9 mg in 100 g cheese.Článekpeer-reviewedpublished Otevřený přístup Multi-elemental analysis of wine samples using ICP-OES and ICP-MS methods. Anthropogenic gadolinium in wine(University of Pardubice, 2021) Mišíková, Frederika; Patočka, Jan; Krejčová, Anna; Adam, MartinThe study presented in this article has been focused on developing a method for elemental analysis of wine samples. A wide sample set of 200 wines contained white, rosé and red still wines; the wine vintages being 2013, 2014, 2015, 2016, 2017 and 2019. Samples were taken from wine festivals in Pardubice and Hradec Králové and private wineries in the Czech Republic. The analysis was performed using inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS); the determined elements being As, Ba, Ca, Cd, Ce, Co, Cu, Dy, Er, Eu, Fe, Gd, Ho, K, La, Lu, Mg, Mn, Na, Nd, Ni, P, Pb, Pr, S, Sm, Sr, Tb, Tm, Y, Yb, and Zn. The data obtained were processed statistically and the Sr/Ba, Sr/Ca and Sr/Mg ratios were determined as potential indicators for the identification and classification of the wine origin. The existence of the so-called gadolinium anomaly for selected wine samples was investigated as evidence of the presence of anthropogenic gadolinium.