23 (2017) Scientific papers, Series A
Permanentní URI k tomuto záznamuhttps://hdl.handle.net/10195/75253
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Článekpeer-reviewedpublisher's version Otevřený přístup 30 years with carbon paste electrodes at the University of Pardubice(University of Pardubice, 2017) Švancara, Ivan; Metelka, Radovan; Mikysek, Tomáš; Vytřas, KarelIn this review (with 425 Refs), three decades of the electrochemistry and electroanalysis with carbon paste electrodes characterising the research activities of the electroanalytical group at the University of Pardubice (EAG UPa) are summarised, when presenting all the achievements and, where applicable, also highlighting research outputs throughout the time. In more detail, reminded are the very beginnings in the mid-1980s and the early era at the University of Chemical Technology. The article contains the complete list of publications plus a great majority of conference presentations associated with the field and having been prepared by the EAG UPa in the period 1987-2016.Článekpeer-reviewedpublisher's version Otevřený přístup Catalitic activity of Agaricus bisporus mushroom tyrosinase to acetaminophenon in the presence of L-proline(University of Pardubice, 2017) Sýs, Milan; Klikarová, Jitka; Česlová, Lenka; Vytřas, KarelSecondary reaction of amino acid L-proline with 4-acetamido-o-benzoquinone as a product of the enzymatic oxidation of acetaminophen mediated by mushroom tyrosinase isolated from Agaricus bisporus has been studied by amperometric detection in the batch injection analysis configuration and by ultraviolet-visible spectrophotometry. Actually, this scientific work is focused on finding out whether it would be possible to develop an indirect electrochemical method based on the amperometric biosensing suitable for monitoring of the L-proline in various honey samples. For optimum content of acetaminophen (100 :mol dm–3) and tyrosinase (3.33 :g cm–3), a satisfactory linear range from 0.1 to 0.5 mmol dm–3 L-proline was obtained for absorption maximum at 332 nm. However, it is necessary to mention that the indirect determination of L-proline by the amperometric tyrosinase biosensor still presents a challenge in the field of developing biological sensors for monitoring of this amino acid.