Zobrazit minimální záznam
dc.contributor.author |
Kanďár, Roman
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dc.contributor.author |
Žáková, Pavla
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dc.date.accessioned |
2020-04-23T08:51:50Z |
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dc.date.available |
2020-04-23T08:51:50Z |
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dc.date.issued |
2009 |
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dc.identifier.isbn |
978-80-7395-239-6 |
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dc.identifier.issn |
1211-5541 |
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dc.identifier.uri |
https://hdl.handle.net/10195/75329 |
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dc.description.abstract |
A method for the measurement of pyridoxal, pyridoxal 5'-phosphate and 4-pyridoxic acid using a high-performance liquid chromatography with fluorescence detection with regard to stability and recovery is described. For the separation, a reverse phase column LiChroCart 125-4, Purospher RP-18e, 5 μm, was used. The mixture of ethanol and 25 mmol/L Na2HPO4 (7:93, v/v), pH 6.0 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked plasma samples were at intervals 93.0–101.8 %, except for pyridoxal (82.5–98.8 %). The limit of detection was 1.0 nmol/L for pyridoxal 5'-phosphate, 0.7 nmol/L for 4-pyridoxic acid and 1.1 nmol/L for pyridoxal. The preliminary reference range of pyridoxal 5'-phosphate, pyridoxal and 4-pyridoxic acid in a group of blood donors are 42.0–75.6 nmol/L, 2.6–12.8 nmol/L and 16.2–33.0 nmol/L, respectively. |
en |
dc.format |
p. 73–85 |
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dc.language.iso |
en |
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dc.publisher |
University of Pardubice |
en |
dc.relation.ispartof |
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical Technology. 15/2009 |
en |
dc.rights |
open access |
en |
dc.title |
Determination of pyridoxal, pyridoxal 5'-phosphate and 4-pyridoxic acid in plasma of selected groups of the patients with a view to proper sample preparation using HPLC with fluorescence detection |
en |
dc.type |
Article |
en |
dc.peerreviewed |
yes |
en |
dc.publicationstatus |
publisher's version |
en |
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