Anticancer potential of Amaryllidaceae alkaloids evaluated by screening with a panel of human cells, real-time cellular analysis and Ehrlich tumor-bearing mice
Článekpeer-reviewedpublishedDatum publikování
2017
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Elsevier Ireland Ltd
Abstrakt
In this study, twenty-two Amaryllidaceae alkaloids were screened for their anticancer potential. All isolates were evaluated for antiproliferative activities on a panel of 17 human cell types of different tissue origin using WST-1 assay. In addition, we determined the antiproliferative effect with a real-time cell analysis xCELLigence system. Thereafter, to evaluate the barely known in vivo anticancer potential of the most potent molecule haemanthamine, a preliminary study was performed using an Ehrlich tumor-bearing mice model. The results showed that haemanthamine, lycorine and haemanthidine exerted the highest antiproliferative activity. The mean growth percent (GP) value after a single-dose 10 mu M treatment was for haemanthamine 21%, for lycorine 21% and for haemanthidine 27% that of untreated control cells (100%). Furthermore, haemanthamine, lycorine and haemanthidine exhibited significant cytotoxicities against all the tested cell lines with individual IC50 values in the micromolar range. Dynamic real-time measures of impedance by xCELLigence indicated that these three compounds suppress cell proliferation after 10 h of treatment at a concentration of 10 mM or higher. Regrettably, in a follow-up in vivo antitumor activity study, haemanthamine showed no statistically significant reduction in the tumor size with no prolongation of survival time of Ehrlich tumor-bearing mice. Taken together, these results provide a new clue and guidance for exploiting Amaryllidaceae alkaloids as anticancer agents.
Rozsah stran
p. 121-132
ISSN
0009-2797
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Chemico-biological Interactions, volume 275, issue: 25 September
Vydavatelská verze
http://www.sciencedirect.com/science/article/pii/S0009279717306713?via%3Dihub
Přístup k e-verzi
Práce není přístupná
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
Umístění tištěné verze
Přístup k tištěné verzi
Klíčová slova
Antiproliferative activity, Amaryllidaceae, Alkaloids, In vitro, In vivo, Antiprolifeační aktivita, Amaryllidaceae, Alkaloidy, In vitro, In vivo