Isotachophoretic Determination of Amino Acids After Their Conversion to Hydroxy Acids
Konferenční objektpeer-reviewedpublishedSoubory
Datum publikování
2017
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Univerzita Karlova. Přírodovědecká fakulta
Abstrakt
Determination of amino acids by capillary isotachophoresis is presented. Developed method is suitable for separation of amino acids from relatively simple mixtures, especially for dietary supplements. The most of amino acids is not possible to separate directly by isotachophoresis. Thus, they had to be converted to other compounds. Chemical conversion to corresponding hydroxy acids in presence of nitrite acid was used. This necessary nitrite acid was prepared by reaction of sodium nitrite with acetic acid. Under optimization, concentration 0.1 mol/L sodium nitrite and acetic acid was found. As the best electrolyte system for separation of hydroxy acids, 0.01 mol/L HCl, 0.05% hydroxyethyl cellulose, with β-alanine (leading electrolyte of pH=3.6) and 0.01 mol/L valeric acid with sodiumhydroxide (terminating electrolyte of pH=7.24) were chosen.
Rozsah stran
p. 20-26
ISSN
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Projekt
Zdrojový dokument
13th ISC Modern Analytical Chemistry
Vydavatelská verze
http://web.natur.cuni.cz/analchem/isc/isc13.pdf
Přístup k e-verzi
open access
Název akce
13th ISC Modern Analytical Chemistry (20.09.2017 - 21.09.2017, Praha)
ISBN
978-80-7444-052-6
Studijní obor
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Klíčová slova
amino acids, hydroxy acids, isotachophoresis, aminokyseliny, hydroxykyseliny, izotachoforéza