New Interface for Purification of Proteins: 1D TiO2 Nanotubes Decorated by Fe3O4 nanoparticles
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Datum publikování
2017-08-08
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American Chemical Society
Abstrakt
In this work, a high surface area interface, based on anodic 1D TiO2 nanotubes homogenously decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs) is reported for the first time for an unprecedented purification of His-tagged recombinant proteins. Excellent purification results were achieved from the model protein mixture, as well as from the whole cell lysate (with His-tagged ubiquitin). Compared to a conventional Immobilized-Metal Affinity Chromatography (IMAC) system, specific isolation of selected His-tagged proteins on behalf of other proteins was significantly enhanced on TiO2NTs@Fe3O4NP´s interface under optimized binding and elution conditions. The combination of specific isolation properties, magnetic features, biocompatibility, and ease of preparation of this material consisting of two basic metal oxides, makes it a suitable candidate for future purification of recombinant proteins in biotechnology. The principally new material bears a large potential to open new pathways for discoveries in nanobiotechnology and nanomedicine.
Rozsah stran
p. 1-41
ISSN
1944-8244
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Projekt
EC/H2020/638857/EU/Towards New Generation of Solid-State Photovoltaic Cell: Harvesting Nanotubular Titania and Hybrid Chromophores/CHROMTISOL
Zdrojový dokument
ACS Applied Materials & Interfaces. 2017
Vydavatelská verze
https://pubs.acs.org/doi/abs/10.1021/acsami.7b08445
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Attribution-NonCommercial-NoDerivs 3.0 Czech Republic
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self-organized TiO2 nanotubes, magnetic nanoparticles, recombinant His-tagged proteins, isolation, purification
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Except where otherwised noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Czech Republic