The purpose of this paper is to demonstrate how efficiently the regions of the Czech Republic used their environmental resources. The quest for sustainability has led to the development of various tools and techniques to measure the progress of on-going efforts. These tools and techniques used for the measurement of sustainability are still evolving. Sustainable Value approach is another simple tool for measuring sustainability performance which integrates the three dimensions of sustainability: the economic development, environmental sustainability and social development. The sustainable value approach assesses how efficiently organizational entities (in this study: regions) use their resources. This paper uses the principles of the sustainable value approach to estimate how the regions of the Czech Republic utilised their environmental resources.