Determination of selected fatty acids in dried sweat spot using gas chromatography with flame ionization detection
ČlánekOmezený přístuppeer-reviewedpublished versionDatum publikování
2016
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Method is described for the determination of fatty acids in dried sweat spot and plasma samples using gas chromatography with flame ionization detection. Plasma and dried sweat spot samples were obtained from a group of blood donors. The sweat was collected from each volunteer during exercise. Sweat was spotted onto collection paper containing butylated hydroxytoluene. Fatty acids were derivatized with acetyl chloride in methanol to form methyl esters of fatty acids. The fatty acids in dried sweat spot samples treated with butylated hydroxytoluene and stored at –20°C were stable for 3 months. Our results indicate that sweat contains, among fatty acids with short chain, also fatty acids with long chain and unsaturated fatty acids. Linear relationships between percentage content of selected fatty acids in dried sweat spot and plasma were observed.
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p. 4377-4383
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1615-9306
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Journal of Separation Science, volume 39, issue: 22
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dried sweat spot, fatty acids, flame ionization detection, gas chromatography, human sweat, suchá kapka potu, mastné kyseliny, plamenově-ionizační detekce, plynová chromatografie, lidský pot