Electrochemical determination of activity of acetylcholinesterase immobilized on magnetic particles
ČlánekOtevřený přístuppeer-reviewedpublished versionDatum publikování
2016
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Acetylcholinesterase (AChE) is an enzyme which terminates action of neurotransmitter acetylcholine in cholinergic system. Activity of enzyme is commonly assayed by Ellman´s method which has significant disadvantages like instability of reagents. Inability to use enzyme from the mixture repeatedly is another disadvantage. Therefore, we developed process using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) to immobilize AChE on the surface of magnetic particles, which enable to separate enzyme and use it repeatedly. To determinate activity of enzyme, we optimized square wave voltammetry (SWV) method where screen-printed sensor with Prussian blue (PB) modified working electrode was performed. We report limit of detection equal 8.1 μM for a tested inhibitor of AChE tacrine. Comparing to the standard Ellman´s test, the immobilized enzyme has limited sensitivity to interferences caused by organic solvents. The proposed method is readily to practical application.
Rozsah stran
p. 4840-4849
ISSN
1452-3981
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International Journal of Electrochemical Science, volume 11, issue: 6
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http://www.electrochemsci.org/papers/vol11/110604840.pdf
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open access
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Acetylcholinesterase, tacrine, magnetic particles, screen-printed electrode, square wave voltammetry, Acetylcholinesterasa, takrin, magnetické částice, tištěné elektrody, square wave voltametrie