Complete fatty acid composition of selected Aspergillus species
ČlánekOtevřený přístuppeer-reviewedpublishedDatum publikování
2006
Vedoucí práce
Oponent
Název časopisu
Název svazku
Vydavatel
Univerzita Pardubice
Abstrakt
The goal of this work was to perform a detailed analysis of fatty acids from Aspergillus species in a search for biomarkers to differentiate the spicies. In the course of this work, we isolated various classes of lipids (neutral lipids, glycolipids, phospholipids and lipopolysaccharides) from six Aspergillus species by chloroform/methanol extraction and multiple column fractionations. Further separation of structurally different fatty acids from phospholipids was also performed. The identities of isolated fatty acids from all lipid fractions were tentatively assigned using gas chromatography/mass spectrometry (GC/MS). The experimental methodology was found to be suitable for complete characterization of the fatty acids from various cellular sources. While the methods used in this work are standard techniques, the detal obtained in the fatty acid composition of each of the Aspergillus species is much greater than previously described.
Rozsah stran
p. 45-58
ISSN
1211-5541
Trvalý odkaz na tento záznam
Projekt
Zdrojový dokument
Scientific papers of the University of Pardubice. Series A, Faculty of Chemical technology. 11(2005)
Vydavatelská verze
Přístup k e-verzi
open access
Název akce
ISBN
Studijní obor
Studijní program
Signatura tištěné verze
47333
Umístění tištěné verze
Univerzitní knihovna (studovna)
Přístup k tištěné verzi
Klíčová slova
mastné kyseliny, Aspergillus spp., Aspergillus niger, analytické metody, plynová chromatografie, Hmotnostní spektrometrie